Recognition of Endogenous Ligands by C-Type Lectins:Interaction of Serum Mannan-binding Protein with Tumor-associated Oligosaccharide Epitopes

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Recognition of Endogenous Ligands by C-Type Lectins:Interaction of Serum Man- nan-binding Protein with Tumor-associated Oligosaccharide Epitopes

Animal lectins have contributed greatly to understanding of the physiological significance of glycans in man and animals. Mannan-binding protein (MBP) binds to mannose, N-acetylglucosamine and L-fucose via the carbohydrate binding sites in its carbohydrate recognition domain (CRD). In pathogenic microorganisms, manno-oligosaccharides on the cell surface appear to be the major glycans involved i...

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Characterization of oligosaccharide ligands expressed on SW1116 cells recognized by mannan-binding protein. A highly fucosylated polylactosamine type N-glycan.

Mannan-binding protein (MBP) is a C-type serum lectin and activates complement through the lectin pathway when it binds to ligand sugars such as mannose, N-acetylglucosamine, and fucose on microbes. In addition, the vaccinia virus carrying the human MBP gene was shown to exhibit potent growth inhibitory activity toward human colorectal carcinoma, SW1116, cells in nude mice. We have proposed cal...

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Mannan-binding protein, a C-type serum lectin, recognizes primary colorectal carcinomas through tumor-associated Lewis glycans.

Mannan (mannose)-binding protein (MBP) is a C-type serum lectin that plays a key role in innate immunity. MBP forms large multimers (200-600 kDa) and exhibits broad specificity for mannose, N-acetylglucosamine, and fucose. MBP exhibits high affinity for unique oligosaccharides that have been isolated from human colorectal carcinoma (SW1116) cells and characterized as highly fucosylated high m.w...

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The mechanism of carbohydrate-mediated complement activation by the serum mannan-binding protein.

Serum mannan-binding protein (S-MBP), a lectin specific for mannose and N-acetylglucosamine, was documented to activate complement through the classical pathway. In this study, we examined the mechanism that initiates this activation. By a passive hemolysis test using sheep erythrocytes coated with yeast mannan, the activation of complement by human S-MBP was shown to proceed in the absence of ...

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ژورنال

عنوان ژورنال: Trends in Glycoscience and Glycotechnology

سال: 2010

ISSN: 0915-7352,1883-2113

DOI: 10.4052/tigg.22.141